Journal: American Journal of Translational Research
Article Title: CoQ10 suppression of oxidative stress and cell senescence increases bone mass in orchiectomized mice
doi:
Figure Lengend Snippet: The effect of CoQ10 on the osteoblastic bone formation in ORX mice. (A) Representative photomicrographs of paraffin sections of tibias from 48-week-old mice of each group stained for HE. (B) The number of osteoblasts (N.Ob) per mm2 trabecular area (T. Area) was measured and presented. (C) Representative photomicrographs of paraffin sections of tibias from 48-week-old mice of each group stained immunohistochemically for type I collagen. (D) The percentage of the Col I-positive area was measured and presented by image analysis (%). Real-time RT-PCR was performed on bone tissue extracts from 48-week-old mice of each group. Gene expression of (E) OPG, (F) RUNX2 and (G) OCN are shown. Messenger RNA expression, assessed by real-time RT-PCR analysis, was calculated as a ratio to the GAPDH mRNA level and expressed relative to levels in sham mice. Data are presented as the mean ± SEM of determinations, each data point is the mean of five specimens. *P < 0.05, ***P < 0.001, versus sham mice; #P < 0.05, ##P < 0.01 versus ORX mice.
Article Snippet: Immunohistochemical staining was carried out for type I collagen, β-galactosidase (β-gal) and p16 INK4a using the avidin-biotin-peroxidase complex technique with affinity-purified goat anti-rabbit type I collagen antibody (Southern Biotechnology Associates, Birmingham, AL, USA), β-gal (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and p16 INK4a (Abcam, MA, USA) following previously-described methods [ 4 ].
Techniques: Staining, Quantitative RT-PCR, Expressing, RNA Expression